Discrimination of Three Amphistome Species by PCR-RFLP Based on rDNA ITS2 Markers
نویسندگان
چکیده
منابع مشابه
Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)
BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...
متن کاملPCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA
Background and Purpose: The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. Materials and Methods: In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fus...
متن کاملmolecular identification and differentiation of fasciola isolates using pcr- rflp method based on internal transcribed spacer (its1, 5.8s rdna, its2)
background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...
متن کاملGenomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP
The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry. Despite efforts to control this disease, the high frequency of new epizootic events indicate that the vaccine and antibiotics treatments have limited effectiveness, therefore the preve...
متن کاملPCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA
BACKGROUND AND PURPOSE The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. MATERIALS AND METHODS In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fus...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2003
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.65.931